| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cano, William (15)
|
MAUN |
48.71 |
49.16 |
0.45 |
| 2 |
Ye, Brandon Z (16)
|
MAGRA |
49.98 |
49.27 |
-0.71 |
| 3 |
Humphrey, Zach D (17)
|
MAGRA |
54.51 |
51.44 |
-3.07 |
| 4 |
Gorman, Ronan M (18)
|
MAGRA |
51.63 |
53.23 |
1.60 |
| 5 |
Arjona, Alexander J (15)
|
MANSC |
54.55 |
53.54 |
-1.01 |
| 6 |
Tebo, Tyler V (16)
|
MAGRA |
53.81 |
54.89 |
1.08 |
| 7 |
Muthaiyan, Pranav D (16)
|
MAWA |
54.90 |
55.07 |
0.17 |
| 8 |
Tangorra, Beppe L (15)
|
MARKRY |
57.15 |
55.58 |
-1.57 |
| 9 |
Overman, Adam T (15)
|
MAFWAC |
55.11 |
55.81 |
0.70 |
| 10 |
Lesher, Charlie M (15)
|
MAGRA |
56.60 |
55.87 |
-0.73 |
| 11 |
Zabigalivk, Maksym (16)
|
MANSC |
56.91 |
56.68 |
-0.23 |
| 12 |
Bustard, Nathaniel S (15)
|
MARKRY |
|
56.89 |
- |
| 13 |
DeHaven, Regan J (16)
|
MAWA |
56.26 |
58.45 |
2.19 |
| 14 |
Cohn, Joshua B (16)
|
MAACAC |
1:01.22 |
1:01.47 |
0.25 |
| 15 |
Srinivasan, Siddarth (15)
|
MAWA |
1:03.18 |
1:03.26 |
0.08 |